Liquid Formulation of Methylene Blue: Characterization and In Vivo Evaluation Against Canine Fibrosarcoma
Keywords:
photodynamic therapy, methylene blue, fibrosarcoma, dogAbstract
Due to the high prevalence of fibrosarcoma, a neoplasm that affects connective tissue in dogs, it is imperative to explore alternative therapies that offer better results and reduce adverse effects. In this context, a liquid formulation of the photosensitizer methylene blue was developed and characterized. This formulation was then evaluated in the in vivo treatment of fibrosarcoma in dogs. To this end, a mixed-breed female dog was subjected to photodynamic therapy protocols. The results showed a significant reduction in the tumor, indicating that the therapy not only improved the clinical condition but also contributed to the dog's well-being and quality of life.
References
ALGORRI, J. F. et al. Photodynamic Therapy: A Compendium of Latest Reviews. Cancers (Basel), v. 13, n. 17, p. 4447, 2021. DOI: 10.3390/cancers13174447.
ALVAREZ, N.; SEVILLA, S. Current Advances in Photodynamic Therapy (PDT) and the Future Potential of PDT-Combinatorial Cancer Therapies. MDPI, v. 25, n. 2, p. 1023, 2024. DOI: 10.3390/ijms25021023.
AL-OBAIDY, R. et al. Targeted Delivery of Paclitaxel Drug Using Polymer-Coated Magnetic Nanoparticles for Fibrosarcoma Therapy: In Vitro and In Vivo Studies. Scientific Reports, [s. l.], 2023.
BELEKOV, E. et al. Improved Antimicrobial Properties of Methylene Blue Attached to Silver Nanoparticles. Photodiagnosis Photodyn Ther, 2020. DOI: 10.1016/j.pdpdt.2020.102012.
CARDOSO, I.; PINELLO, K.; RIBEIRO, J. Animal Oncology Registry. Veterinary Oncology Network, [s. l.], v. 1, ed. 1, April 2021.
CARDOZO, A. P. Use of Antimicrobial Photodynamic Therapy (aPDT) with Methylene Blue in Animal Model Studies: A Systematic Review. Universidade Nove de Julho, [s. l.], 2021.
CORREIA, J. Photodynamic Therapy Review: Principles, Photosensitizers, Applications and Future Directions. Pharmaceutics, v. 13, n. 9, p. 1332, 2021. DOI: 10.3390/pharmaceutics13091332.
DALECK, C. et al. Neoplasms of the Tongue in Five Dogs. Ciência Rural, [s. l.], v. 37, n. 2, p. 578-582, April 2007.
EMIRDOVER, C. et al. An Epidemiological Analysis and Evaluation of 45 Patients Between 2006-2021: A Descriptive Study of Rare Tumor. Original Article, [s. l.], Dec. 26, 2023.
FERNANDES, C. et al. Recurrence of Application of Fibrosarcoma in an Elderly Cat and Multimodal Treatment of Surgical Excision and Intraoperative Electrochemotherapy (Felis catus domesticus): Case Report. Brazilian Journal of Development, [s. l.], v. 9, n. 1, p. 5621-5636, Jan. 26, 2023.
HU, H. et al. Polyethylene Glycol Modified Protamine-Chlorin e6 Conjugate Nanoparticles for Enhanced Cancer Photodynamic Therapy. Elsevier, [s. l.], Feb. 22, 2024.
KITAI, R. et al. Successful Treatment of Fibrosarcoma in the Occipital Bone: A Case Report. Elsevier, [s. l.], Apr. 21, 2021.
LAKI, C. et al. Effects of Distance Vibration in Quality of Life and Clinical Stability of Canine Patients Submitted to Oncological Treatment. Cadernos de Naturologia e Terapias Complementares, [s. l.], Nov. 3, 2022.
MACHADO, G. Treatment of Soft Tissue Sarcoma in Dogs: A Literature Review. Faculdade de Júlio de Mesquita Filho, [s. l.], 2022.
MARTINS, D. Antimicrobial Photodynamic Therapy in the Treatment of Canine Otitis. Universidade Brasil, [s. l.], 2023.
PEREIRA, F. Evaluation of Collagen-Based Dressings with Photoactive Drugs for Post-Surgical Photodynamic Therapy In Vivo. Institute of Energy and Nuclear Research, [s. l.], 2022.
RADAK, M.; GHAMARI, N.; FALLAHI, H. Identification of Common Factors Among Fibrosarcoma, Rhabdomyosarcoma, and Osteosarcoma by Network Analysis. Elsevier, [n.p.], Dec. 3, 2023.
SOUZA, M. V. et al. Applicability of Electrochemotherapy in Veterinary Medicine: Literature Review. Revista Terra e Cultura, [s. l.], v. 39, 2023.
SPINOSA, H. S.; GÓRNIAK, S. L.; BERNARDI, M. M. Pharmacology Applied to Veterinary Medicine. 2006.
TALDAEV, A. et al. Methylene Blue in Anticancer Photodynamic Therapy: Systematic Review of Preclinical Studies. Front Pharmacol, 2023. DOI: 10.3389/fphar.2023.1264961.
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