MICROBIOLOGICAL AND PHYSICOCHEMICAL ANALYSIS OF HOMEMADE FRESH MILK MARKETED IN ANÁPOLIS - GO
Palavras-chave:
Physicochemical analysis, Salmonella spp., Raw milkResumo
Title:
Microbiological and Physicochemical Analysis of Homemade Fresh Milk Marketed in Anápolis - GO
Objective:
To analyze the microbiological and physicochemical quality of homemade fresh milk sold in the Anápolis - GO region, focusing on the detection of Salmonella spp.
Justification:
Raw milk consumption is common in family and rural settings, often without proper sanitary inspection, posing a public health risk due to potential contamination with pathogenic microorganisms.
Methodology:
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Sampling: Milk samples collected over one year.
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Physicochemical analyses: pH, titratable acidity, density, fat content, protein content, cryoscopy, and water activity.
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Microbiological analyses: Mesophilic bacteria count, total and thermotolerant coliforms, molds and yeasts, and detection of Salmonella spp. using selective media and confirmatory tests.
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Methodological references: AOAC, Embrapa, MAPA, and ANVISA.
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Data comparison: Results will be compared with MAPA Normative Instruction No. 76/2018 and ANVISA Normative Instruction No. 161/2022.
Expected Results:
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Variations in physicochemical parameters among samples.
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Presence of Salmonella spp. in some samples.
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Evidence of deficiencies in milking and milk preservation practices.
Impact and Applications:
The findings may support educational initiatives and hygienic-sanitary control strategies, promoting safer artisanal milk production and consumption.
Referências
ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS – AOAC. Official methods of analysis of the AOAC International. 21st ed. Gaithersburg, MD: AOAC International, 2019.
BONFOH, B., WASEM A., TRAORÉ A.N., FANÉ A., SPILLMANN H., SIMBÉ C.F., ALFAROUKH I.O., NICOLET J., FARAH Z., ZINSSTAG J. Microbiological quality of cow ́s milk taken at different intervals from the udder to selling point in Bamako (Mali). Food Control, v. 14, n. 7, p. 495-500, 2003
BRASIL. Agência Nacional de Vigilância Sanitária. Instrução Normativa nº 161, de 1º de julho de 2022. Estabelece os padrões microbiológicos de alimentos. Diário Oficial da União: seção 1, Brasília, DF, p. 122, 04 jul. 2022. Disponível em: https://www.in.gov.br/en/web/dou/-/instrucao-normativa-n-161-de-1-de-julho-de-2022-412051908. Acesso em: 25 abr. 2025.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa n.º 76, de 26 de novembro de 2018. Dispõe sobre os requisitos para produção de leite cru refrigerado, pasteurizado e cru tipo A. Diário Oficial da União: seção 1, Brasília, DF, n. 230, p. 6-12, 30 nov. 2018. Disponível em: https://www.in.gov.br/materia/-/asset_publisher/Kujrw0TZC2Mb/content/id/55486621. Acesso em: 25 abr. 2025.
